Faculty of Science

Department of Pharmacy

scientific publication


 international peer reviewed article | editorial | book chapter | media coverage

 2015 | 2014 | 2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2000-2005 |1998-1999


  1. YJ Hong, YMF Chia, RH Yeo, G Venkatesan, SK Koh, CLL Chai, L Zhou, P Kojodjojo and ECY Chan*. Inactivation of human cytochrome P450 3A4 and 3A5 by dronedarone and N-desbutyl dronedarone. Molecular Pharmacology DOI: 10.1124/mol.115.100891 In Press. New!
  2. LC Phua, CH Wilder-Smith, YM Tan, T Gopalakrishnan, RK Wong, X Li, ME Kan, J Lu, A Keshavarzian and ECY Chan*. Gastrointestinal symptoms and altered intestinal permeability induced by combat-training are associated with distinct metabotypic changes. Journal of Proteome Research 14 (October 2015) 4734 - 4742. New!
  3. P Bahety, THV Nguyen, YJ Hong, L Zhang, ECY Chan* and PL Ee*.Understanding the cholesterol metabolism-perturbing effects of docosahexaenoic acid by gas chromatography-mass spectrometry targeted metabonomic profiling. European Journal of Nutrition DOI: 10.1007/s00394-015-1053-4 In Press. New!
  4. LY Yip and ECY Chan*. Investigation of Host-Gut Microbiota Modulation of Therapeutic Outcome. Drug Metabolism and Disposition 43 (October 2015) 1619-1631 DOI: 10.1124/dmd.115.063750 New!
  5. CY Chan, L Zhou and ECY Chan*. The Isotope-Coded Affinity Tag Method for Quantitative Protein Profile Comparison and Relative Quantitation of Cysteine Redox Modification. Current Protocols in Protein Science In Press. New!
  6. WYB Foo, HY Tay, ECY Chan and AJ Lau*. Meclizine, a pregnane X receptor agonist, is a direct inhibitor and mechanism-based inactivator of human cytochrome P450 3A. Biochemical Pharmacology 97 (October 2015) 320-330. DOI:10.1016/j.bcp.2015.07.036 New!
  7. LC Phua, HW Ng, AHL Yeo, E Chen, MSM Lo, PY Cheah, ECY Chan, PK Koh* and HK Ho*. Prevalence of KRAS, BRAF, PI3K and EGFR mutations among Asian metastatic colorectal cancer patients. Oncology Letters 10 (October 2015) 2519-2526. DOI: 10.3892/ol.2015.3560  New!
  8. LY Chen, J Li, T Guo, S Ghosh, SK Koh, D Tian, L Zhang, D Jia, RW Beuerman, R Aebersold, ECY Chan*, L Zhou*. Global metabonomic and proteomic analysis of human conjunctival epithelial cells (IOBA-NHC) in response to hyperosmotic stress. Journal of Proteome Research 14 (August 2015) 3982-3995. DOI: 10.1021/acs.jproteome.5b00443
  9. TP Lim, YY Cai, YJ Hong, ECY Chan, S Suranthran, J Teo, W Lee, TY Tan, LY Hsu, TH Koh, TT Tan and AL Kwa*. In-vitro Pharmacodynamics of Various Antibiotics in Combination against Extensively Drug-Resistant Klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy 59 (May 2015) 2515-2524.
  10. ECY Chan*, KK Pasikanti, YJ Hong, PC Ho, R Mahendran, LRN Mani, E Chiong and K Esuvaranathan*. Metabonomic profiling of bladder cancer. Journal of Proteome Research 14 (February 2015) 587-602.
  11. HK Ho, CY Chan, KD Hardy and ECY Chan. Mechanism-based inactivation of CYP450 enzymes: a case study of lapatinib. Drug Metabolism Review 47 (February 2015) 21-28.
  12. KL Chang, HN Pee, WP Tan, GS Dawe, E Holmes, JK Nicholson, ECY Chan and PC Ho*. Metabolic profiling of CHO-AßPP695 cells revealed mitochondrial dysfunction prior to Aß pathology and potential therapeutic effects of both PPARγ and PPARα agonisms for Alzheimer’s disease. Journal of Alzheimer's Disease 44 (January 2015) 215-231. 



  1. LC Phua, XP Chue, PK Koh, PY Cheah, ECY Chan* and HK Ho*. Global fecal microRNA profiling in the identification of biomarkers for colorectal cancer screening among Asians.Oncology Reports 32 (May 2014) 97-104
  2. LC Phua, XP Chue, PK Koh, PY Cheah, HK Ho* and ECY Chan*. Non-invasive fecal metabonomic detection of colorectal cancer. Cancer Biology & Therapy 15 (April 2014) 389-397.
  3. MD Damavandi, ECY Chan, JP Kraus, PC Ho and TS Kang*. Development of an UPLC-MS/MS method for assaying the enzymatic activity of propionyl coenzyme-A carboxylase. Bioanalysis 6 (February 2014) 335-348.
  4. P Bahety, YM Tan, YJ Hong, L Zhang, ECY Chan* and PL Ee*. Metabotyping of docosahexaenoic acid - treated Alzheimer’s disease cell model. PLOS ONE (February 2014) DOI: 10.1371/journal.pone.0090123.
  5. T Nema, ECY Chan, PC Ho*. Applications of monolithic materials for sample preparation.Journal of Pharmaceutical and Biomedical Analysis 87 (January 2014) 130-141.
  6. EA Bard-Chapeau, A Nguyen, AG Rust, A Sayadi, P Lee, BQ Chua, LS New, J de Jong, JM Ward, CKY Chin, V Chew, HC Toh, J Abastado, T Benoukraf , R Soong, FA Bard, AJ Dupuy, RL Johnson, GK Radda, ECY Chan, LFA Wessels, DJ Adams, NA Jenkins and NG Copeland*. Transposon mutagenesis identifies genes driving hepatocellular carcinoma in a chronic hepatitis B mouse model. Nature Genetics 46 (January 2014) 24-32.


  1. LC Phua, PK Koh, PY Cheah, HK Ho* and ECY Chan*. Global gas chromatography/time-of-flight mass spectrometry (GC/TOFMS)-based metabonomic profiling of lyophilized human feces. Journal of Chromatography B 937 (October 2013) 103-113.
  2. KK Pasikanti, K Esuvaranathan,YJ Hong, PC Ho, R Mahendran, LRN Mani, E Chiong and ECY Chan*. Urinary metabotyping of bladder cancer using two-dimensional gas chromatography time-of-flight mass spectrometry. Journal of Proteome Research 12 (September 2013) 3865-3873.
  3. BD Prakash, K Esuvaranathan,PC Ho, KK Pasikanti, ECY Chan and CW Yap*. An automated Pearson's correlation change classification (APC3) approach for GC/MS metabonomic data using total ion chromatogram (TIC). Analyst 138 (May 2013) 2883-2889. 
  4. HH Cheong, J Masilamani, ECY Chan, SY Chan* and TT Phan*. Metabolically functional hepatocyte-like cells from human umbilical cord lining epithelial cells. Assay and Drug Development Technologies 11 (March 2013) 130-138.
  5. LC Phua, M Mal, PK Koh, PY Cheah, ECY Chan* and HK Ho*. Investigating the roles of nucleoside transporters in the resistance of colorectal cancer to 5-fluorouracil therapy. Cancer Chemotherapy and Pharmacology 71 (March 2013) 817-823.
  6. LS New, TP Lim, JW Oh, GJS Cheah, AL Kwa and ECY Chan*. Optimizing hollow-fiber-based pharmacokinetic assay via chemical stability study to account for inaccurate simulated clearance of rifampicin. Analytical and Bioanalytical Chemistry 405 (4) (January 2013) 1407-1415.


  1. ZP Hu, ER Browne, T Liu, TE Angel, PC Ho and ECY Chan*. Metabonomic profiling of TASTPM transgenic Alzheimer's disease mouse model. Journal of Proteome Research 11 (December 2012) 5903-5913.
  2. S Saha, DSZ Chan, CY Lee, W Wong, LS New, WK Chui, ECY Chan* and HK Ho*. Pyrrolidinediones reduce the toxicity of thiazolidinediones and modify their anti-diabetic and anti-cancer properties. European Journal of Pharmacology 697 (December 2012) 13-23.
  3. YL Teo, M Saetaew, S Chanthawong, YS Yap, ECY Chan, HK Ho and A Chan*. Effect of CYP3A4 inducer dexamethasone on hepatotoxicity of lapatinib: clinical and in vitro evidence. Breast Cancer Research and Treatment 133 (June 2012) 703-711.
  4. WPK Ching, SH Thng, AP Hui, DY Lee, ECY Chan* and YS Ho*. LC-MS-based metabolic characterization of high monoclonal antibody-producing chinese hamster ovary cells. Biotechnology and Bioengineering 109 (December 2012) 3103-3111.
  5. ECY Chan*, LS New, TB Chua, CW Yap, HK Ho and SD Nelson. Interaction of lapatinib with cytochrome P450 3A5. Drug Metabolism & Disposition 40 (July 2012) 1414-1422.
  6. HT Chng, HK Ho, CW Yap, SH Lam and ECY Chan*. An investigation of the bioactivation potential and metabolism profile of zebrafish versus human. Journal of Biomolecular Screening 17 (August 2012) 974-986.
  7. ECY Chan*. Thomas O. Metz (Ed.): Metabolic profiling. Methods and protocols. Analytical Bioanalytical Chemistry 403 (July 2012) 2465-2466.
  8. M Mal, PK Koh, PY Cheah and ECY Chan*. Metabotyping of human colorectal cancer using two-dimensional gas chromatography mass spectrometry. Analytical Bioanalytical Chemistry 403 (April 2012) 483-493.


  1. T Nema, ECY Chan and PC Ho*. Efficiency of a miniaturized silica monolithic cartridge in reducing matrix ions as demonstrated in the simultaneous extraction of morphine and codeine from urine samples for quantification with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Journal of Mass Spectrometry 46 (September 2011) 891-900.
  2. LY Chen, L Zhou*, ECY Chan*, J Neo and R Beuerman. Characterization of the human tear metabolome by LC-MS/MS. Journal of Proteome Research 10 (October 2011) 4876-4882.
  3. CY Chan, LS New, HK Ho and ECY Chan*. Reversible time-dependent inhibition of cytochrome P450 enzymes by duloxetine and inertness of its thiophene ring towards bioactivation. Toxicology Letters 206 (October 2011) 314-324.
  4. KM Banday, KK Pasikanti, ECY Chan, R Singla, KVS Rao, VA Chauhan* and RK Nanda*. Urine volatile organic compounds could discriminate tuberculosis patients from healthy subjects. Analytical Chemistry 83 (2011) 5526-5534.
  5. ECY Chan*, KK Pasikanti and JK Nicholson. Global urinary metabolic profiling procedures using gas chromatography mass spectrometry. Nature Protocols 6 (October 2011) 1483-1499.
  6. T Nema, ECY Chan and PC Ho*. Extraction of ketamine from urine using a miniature silica monolithic cartridge followed by quantification with liquid chromatography tandem mass spectrometry (LC-MS/MS). Journal of Separation Science 10 (2011) 2079-2087.
  7. KL Chang, LS New, M Mal, CW Goh, CC AW, ER Browne and ECY Chan*. Metabolic profiling of 3-nitroproprionic acid early-stage Huntington's disease rat model using gas chromatography time-of-flight mass spectrometry. Journal of Proteome Research 10 (2011) 2079-2087.
  8. H Takakusa, MD Wahlin, C Zhao, KL Hanson, LS New, ECY Chan and SD Nelson*. Metabolite-intermediate complex formation of human cytochrome P450 3A4 by lapatinib. Drug Metabolism & Disposition 39 (2011) 1022-1030.
  9. H Sukardi, HT Chng, ECY Chan, Z Gong and SH Lam*. Zebrafish for drug toxicity screening: bridging the in vitro cell-based models and in vivo mammalian models. Expert Opinion on Drug Metabolism and Toxicology 7 (2011) 579-589.
  10. DF Toh, DN Patel, ECY Chan, A Yeo, SY Neo and HL Koh*. Anti-proliferative effects of raw and steamed extracts of Panax notoginseng and its ginsenoside constituents on human liver cancer cells. Chinese Medicine 6 (2011) 1-9.
  11. M Mal, PK Koh, PY Cheah and ECY Chan*. UPLC/MS/MS targeted profiling of arachidonic acid and eicosanoids in human colorectal cancer. Rapid Communications in Mass Spectrometry 25 (2011) 755-764.
  12. DJY Ng, KK Pasikanti and ECY Chan*. Trend analysis of metabonomics and systemic review of metabonomics-derived cancer marker metabolites. Metabolomics 7 (2011) 155-178.


  1. YT Koh, KK Pasikanti, CW Yap* and ECY Chan*. Comparative evaluation of software for retention time alignment of gas chromatography/time-of-flight mass spectrometry-based metabonomic data. Journal of Chromatography A 1217 (2010) 8308-8316.
  2. KK Pasikanti, J Norasmara, S Cai, R Mahendran, K Esuvaranathan, PC Ho and ECY Chan*. Metabolic footprinting of tumorigenic and non-tumorigenic uroepithelial cells using two-dimensional gas chromatography time-of-flight mass spectrometry. Analytical and Bioanalytical Chemistry 398 (2010) 1285-1293.
  3. WC Teng, JW Oh, LS New, MD Wahlin, SD Nelson, HK Ho and ECY Chan*. Mechanism-based inactivation of cytochrome P450 3A4 by lapatinib. Molecular Pharmacology 78 (2010) 693-703.
  4. T Nema, ECY Chan and PC Ho*. Application of silica based monolith as solid phase extraction cartridge for extracting polar compounds from urine. Talanta 82 (2010) 488-494.
  5. X Ma and ECY Chan*. On-line chromatographic screening of matrix metalloproteinase inhibitors using immobilized MMP-9 enzyme reactor. Journal of Chromatography B 878 (2010) 1777-1783.
  6. KK Pasikanti, K Esuvaranathan, PC Ho, R Mahendran, R Kamaraj, QH Wu, E Chiong and ECY Chan*. Noninvasive urinary metabonomic diagnosis of human bladder cancer. Journal of Proteome Research 9 (2010) 2988-2995.   
  7. S Saha, LS New, HK Ho, WK Chui, ECY Chan*. Direct toxicity effects of sulfo-conjugated troglitazone on human hepatocytes. Toxicology Letters 195 (2010) 135-141.  
  8. DF Toh, LS New, HL Koh* and ECY Chan*. Ultra-high performance liquid chromatography / time-of-flight mass spectrometry (UHPLC/TOFMS) for time-dependent profiling of raw and steamed Panax notoginsengJournal of Pharmaceutical and Biomedical Analysis 52 (2010) 43-50.
  9. S Saha, LS New, HK Ho, WK Chui and ECY Chan*. Investigation of the role of the thiazolidinedione ring of troglitazone in inducing hepatotoxicity. Toxicology Letters 192 (2010) 141-149.
  10. X Ma and ECY Chan*. Fluorescence-based liver microsomal assay for screening of pharmaceutical reactive metabolites using glutathione conjugated 96-well plates. Bioconjugate Chemistry 21 (2010) 46-55.
  11. HT Chng, LS New, AH Neo, CW Goh, ER Browne and ECY Chan*. A sensitive LC/MS/MS bioanalysis assay of orally-administered lipoic acid in rat blood and brain tissue. Journal of Pharmaceutical and Biomedical Analysis 51 (2010) 754-757.


  1. DPQ Goh, AH Neo, CW Goh, CC Aw, LS New, WS Chen, Z Atcha, ER Browne and ECY Chan*. Metabolic Profiling of Rat Brain and Cognitive Behavioral Tasks: Potential Complementary Strategies in Preclinical Cognition Enhancement Research. Journal of Proteome Research 8 (2009) 5679-5690.
  2. R  Kashimshetty, VG Desai, VM Kale, T Lee, CL Moland, WS Branham, LS New, ECY Chan, H Younis and UA Boelsterli*. Underlying mitochondrial dysfunction triggers flutamide-induced oxidative liver injury in a mouse model of idiosyncratic drug toxicity. Toxicology and Applied Pharmacology 238 (2009) 150-159.
  3. M Mal, PK Koh, PY Cheah and ECY Chan*. Development and validation of a gas chromatography mass spectrometry (GC/MS) method for the metabolic profiling of human colon tissue. Rapid Communications in Mass Spectrometry 23 (2009) 487-494.  
  4. ECY Chan*, LS New, CW Yap and LT Goh. Pharmaceutical metabolite profiling using quadrupole/ ion mobility spectrometry/ time-of-flight mass spectrometry. Rapid Communications in Mass Spectrometry 23 (2009) 384-394.
  5. ECY Chan*, PK Koh, M Mal, PY Cheah, KW Eu, A Backshall, R Cavill, JK Nicholson and H Keun. Metabolic profiling of human colorectal cancer using high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy and gas chromatography mass spectrometry (GC/MS). Journal of Proteome Research 8 (2009) 351-361.
  6. HT Chng, LS New, AH Neo, CW Goh, ER Browne and ECY Chan*. Distribution study of orally administered lipoic acid in rat brain tissues. Brain Research 1251 (2009) 80-86.


  1. KK Pasikanti, PC Ho and ECY Chan*. Development and validation of a GC/MS metabonomic platform for the global profiling of urinary metabolites. Rapid Communications in Mass Spectrometry 22 (2008) 2984-2992.
  2. LC Phua, LS New, CW Goh, AH Neo, ER Browne and ECY Chan*. Investigation of the drug-drug Interaction between α-lipoic Acid and valproate via mitochondrial β-oxidation. Pharmaceutical Research 25 (2008) 2639-2649.
  3. WS Law, PY Huang, ES Ong*, CN Ong, SFY Li, KK Pasikanti, ECY Chan. Metabonomics investigation of human urine after ingestion of green tea with gas chromatography/mass spectrometry, liquid chromatography/mass spectrometry and 1H NMR spectroscopy. Rapid Communications in Mass Spectrometry 22 (2008) 2436-2446.
  4. SC Tan, LS New and ECY Chan*. Prevention of acetaminophen (APAP) induced hepatotoxicity by leflunomide via inhibition of APAP biotransformation to N-acetyl-p-benzoquinone imine. Toxicology Letters 180 (2008) 174-181.
  5. QM Seah, LS New, ECY Chan, UA Boelsterli*. Oxidative bioactivation and toxicity of leflunomide in immortalized human hepatocytes and kinetics of the non-enzymatic conversion to its major metabolite, A77-1726. Drug Metabolism Letters 2 (2008) 153-157.
  6. KK Pasikanti, PC Ho and ECY Chan*. Gas Chromatography/Mass Spectrometry in Metabolic Profiling of Biological Fluids. Journal of Chromatography B 871 (2008) 202-211. Top 25 Most Cited Review Article (2008-2009)  
  7. LS New and ECY Chan*.Evaluation of BEH C18, BEH HILIC and HSS T3 (C18) Column Chemistries for the UPLC/MS/MS Analysis of Glutathione, Glutathione Disulfide and Ophthalmic Acid in Mouse Liver and Human Plasma.  Journal of Chromatographic Science 48 (2008) 209-214 (Special UPLC Issue).


  1. ECY Chan* and LS New. In Vitro Metabolism of Leflunomide by Mouse and Human Liver Microsomes. Drug Metabolism Letters 1 (2007) 299-305.  
  2. LS New, S Saha, MMK Ong, UA Boelsterli and ECY Chan*. Pharmacokinetic Study of Intraperitoneal Administered Troglitazone in Mice Using UPLC/MS/MS. Rapid Communications in Mass Spectrometry 21 (2007) 982-988.
  3. ECY Chan*, SL Yap, AJ Lau, PC Leow, DF Toh and HL Koh. UPLC/TOFMS Based Metabolomics of Raw and Steamed Panax notoginseng. Rapid Communications in Mass Spectrometry 21 (2007) 519-528. 



  1. HL Koh*, AJ Lau and ECY Chan.  Hydrophilic interaction liquid chromatography with tandem mass spectrometry for the determination of underivatized dencichine (b-N-oxalyl-L-a,b-diaminopropionic acid) in Panax medicinal plant species. Rapid Communications in Mass Spectrometry 9 ( 2005) 1237-1244.
  2. ECY Chan*, TW Lee, PC Ho and LJ Fang. Modeling Caco-2 permeability of drugs using immobilized artificial membrane chromatography and physicochemical descriptors. Journal of Chromatography A 1072 (2005) 159-168. 
  3. L Bo and ECY Chan*. Application of visual basic in high-throughput mass spectrometry-directed purification of combinatorial libraries. Journal of Combinatorial Chemistry 5 (2003) 834-841.
  4. ECY Chan and PC Ho*. Preparation and characterization of immunogens for antibody production against metanephrine and normetanephrine. Journal of Immunological Methods 266 (2002) 143-154. 
  5. ECY Chan*, AW Wang, WP Jia and GD Dollinger. An automated LC method for the small-scale purification of organic molecules derived from combinatorial libraries.Journal of Pharmaceutical and Biomedical Analysis 29 (2002) 139-146.
  6. ECY Chan and PC Ho*. High-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometric method for the analysis of catecholamines and metanephrines in human urine. Rapid Communications in Mass Spectrometry 14 (2000) 1959-1964.
  7. ECY Chan, PY Wee, PY Ho and PC Ho*. High-performance liquid chromatographic assay for catecholamines and metanephrines using fluorimetric detection with pre-column 9-fluorenylmethyloxycarbonyl chloride derivatization. Journal of Chromatography B 749 (2000) 179-189
  8. ECY Chan, PY Wee and PC Ho*. Evaluation of degradation of urinary catecholamines and metanephrines and deconjugation of their sulfoconjugates using stability-indicating reversed-phase ion-pair HPLC with electrochemical detection. Journal of Pharmaceutical and Biomedical Analysis 22 (2000) 515-526.
  1. ECY Chan, PY Wee and PC Ho*. The value of analytical assays that are stability-indicating. Clinica Chimica Acta 288 (1999) 47-53. 
  2. ECY Chan and PC Ho*. Enantiomeric separation of tramadol hydrochloride and its metabolites by cyclodextrin-mediated capillary zone electrophoresis. Journal of Chromatography B 707 (1998) 287-294.



  1. YJ Hong, K Esuvaranathan, KK Pasikanti and ECY Chan*. Metabonomic diagnosis of bladder cancer - what are the advantages and considerations? Bioanalysis In Press. New!


 book chapter

  1. LY Yip and ECY Chan*. Gas Chromatography/Mass spectromerty-Based Metabonomics. In: Proteomic and Metabolomic Approaches to Biomarker Discovery. Ed. TD Veenstra and HJ Issaq. Elsevier (June 2013)
  2. ECY Chan*, M Mal and KK Pasikanti. Metabonomics. In: Gas Chromatography. Ed. CF Poole. Elsevier (June 2012) 545-562.
  3. PC Ho and ECY Chan. Chromatographic Measurement of Catecholamines and Metanephrines. In: Chromatographic Methods in Clinical Chemistry and Toxicology. Ed. RL Berholf and RE Winecker. John Wiley & Sons, Ltd. (March 2007) 101-119. 


media coverage

  1. Painless, cheaper way to diagnose bladder cancer. Judith Tan. The Straits Time, 22 May 2010, Sicence, pD10.